Technology behind Molecular Stethoscope

Key developments in molecular biology, bioinformatics and drug development have positioned Molecular Stethoscope to leverage cf-mRNA to create accurate and clinically useful diagnostic tests.

  • Molecular Stethoscope has developed new methods for characterizing highly complex, rare fragments of mRNA in blood
  • Evolution of knowledge around pathways and novel computational techniques for processing and interpreting big data sets has enabled the formation of the Molecular Stethoscope proprietary database
  • Proliferation of expensive and highly effective targeted therapeutics has created an urgent need for diagnostics to facilitate informed selection
mRNA Polymerase Nontemplate Strand Template Stand mRNA Ribonucleotide
  • RNA Isolation

  • Quantification

    Sequencing and qPCR

  • Identification

    Tissue specific gene expression database

  • Separation

  • Algorithm development by machine learning

  • Deconvolution of disease state


Topol E, Quake SR (2015)
A stethoscope for the next 200 years. The Wall Street Journal

Koh W, et al. (2014)
Noninvasive in vivo monitoring of tissue-specific global gene expression in humans. PNAS 111(20):7361–7366.

Damani S, et al. (2012)
Characterization of circulating endothelial cells in acute myocardial infarction. Sci Transl Med. 4(126):126ra33.